By Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki
Animal mobile know-how is a becoming self-discipline of mobile biology which goals not just to appreciate constructions, features and behaviors of differentiated animal cells but additionally to check their skills for use in commercial and scientific reasons. The aim of animal mobilephone know-how contains accomplishments of clonal enlargement of differentiated cells with beneficial skill, optimization in their tradition stipulations, modulation in their skill to provide medically and pharmaceutically, vital proteins, and the appliance of animal cells to gene remedy and synthetic organs. This quantity supplies the readers an entire evaluation of current state-of-the-art in Japan. The lawsuits might be beneficial for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines regarding animal telephone tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
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Extra resources for Animal Cell Technology: Challenges for the 21st Century
Pp. 59-19, Hanser, New York. A. F. (1992) Development ofthe optimal inoculation conditions for microcarrier cultures. Biotechnology and Bioengineering 39,305-3 13. F. ) , pp. 105-138, Academic Press, New York. B. (1988) Overview of cell culture systems and their scale-up in Animal cell biotechnology 3 (Spier, RE. ), pp. 179-220, Academic Press, London. B. and Thornton, B. (1982) Use of microcarrier culture for the production of herpes simplex virus (type 2) in MRC-5 cells. Journal of Chemical Technology andBiotechnology 32,324-329.
2 Effect maintenance phase. of serum concentration on concentration of CHO cells during cell Fig. 3 Effect of serum concentration on specific tPA production rate. Left; cell growth phase, right; cell maintenance phase. Relative value of specific growth rate Fig . 4 Relation between specific rates of cell growth and tPA production. relative value of specific rates against those for the culture using standard medium were employed in each culture experiments. 32 Key words: CHO cells: tPA, Serum, Medium components, Specific metabolic rates References M.
Perfusion culture has the advantages of high volumetric productivity and efficient medium usage. But the process control and long-term operation ofperfusion culture are difficult because of complicate operation system. Recently, many researches have been carried out in the field of process control, and it is possible to cultivate the hybridoma at high density with perfusion. Bleeding the cells is useful strategy to keep a high viability ofthe cell and to prevent from clogging ofthe membrane. YUN ET AL.